陳盈嵐 副教授

學群：農業及海洋生物科技產業學群

研究專長：胜肽訊息傳遞, 質譜技術, 液相層析串聯式質譜儀, 胜肽體學

E-mail：cyl0828@gs.ncku.edu.tw

研究室：89902

研究室Tel：+886-6-2757575#58221

實驗室Tel：+886-6-2757575#58234#913

我們的研究團隊旨在整合基於液相層析串聯式質譜儀的多體學技術，包括蛋白質體學、胜肽體學和代謝體學分析，以揭示植物與線蟲相互作用的胜肽訊號路徑和調控機制。我們發現了一系列在演化上保守的長距離移動胜肽，可調節木質素生物合成和植物免疫，這些胜肽可以進一步應用於線蟲防治上。植物寄生線蟲被認為是農業上重要的害蟲，在全世界造成嚴重的作物和經濟損失。目前關於線蟲管理的策略是作物輪作、土壤滅菌、抗病品系和殺線蟲劑的使用。然而這些方法在寄生線蟲的適應演化過程逐漸變得不那麼有效。高劑量的殺線蟲劑對環境、生態系統和人類健康帶來顯著的負面影響。因此，制定有效和環境友好的線蟲防治策略是必要的。胜肽是細胞間通訊的主要介質，其涉及多細胞生物中的幾乎所有生理過程。目前已知植物胜肽荷爾蒙可以系統性地調節發育過程、非生物和生物逆境反應，並且最近發現宿主模擬胜肽對於寄生線蟲感染十分重要，因此研究胜肽在植物和線蟲相互作用的系統性調節作用不僅促進對植物寄生線蟲感染的生理學知識，也能夠為開發新的線蟲防治生物技術策略的找尋替代前景。

Evolutionarily conserved long-distance migrating peptides Lignin in vascular tissues plays a critical role for water-conducting and structural-supporting functions and also forms physical barriers against pathogen invasion. Comprehensive knowledge has been established on the transcriptional networks for the regulation of lignin biosynthesis. However, the long-distance migrating peptides transported by vascular sap for regulating monolignol biosynthesis remain unknown. Here, we used high throughput peptidomic analyses to reveal a group of evolutionarily conserved long-distance migrating peptides in vascular sap of six plant species, including three woody plants Cinnamomum kanehirae, Populus trichocarpa, Eucalyptus grandis and three crops Solanum lycopersicum (tomato), Glycine max (soybean) and Zea mays (maize). We found one of the conserved peptides, named Angiosperm Sap Peptide (ASAP), can strengthen the plant cell wall through the enhancement of the S-lignin biosynthesis pathway and trigger the salicylic acid-mediated immune responses to resist root-knot nematodes. The phosphoproteomic analyses exhibited that the ASAP rapidly induced protein phosphorylation involved in lignin biosynthesis and xylem development. Sequence identity analysis showed that ASAP emerged in land plants, suggesting its evolutionarily conserved role on regulation of lignin biosynthesis to facilitate plant terrestrialization and resistance to pathogens.