|南加州大學||生化及分子生物研究所||美國||博士||1987.09 ~ 1993.05|
|國立臺灣大學||農化所||中華民國||碩士||1983.09 ~ 1985.06|
|國立臺灣大學||農業化學系||中華民國||學士||1979.09 ~ 1983.06|
My laboratory of biomedical technology (BMT) aims at the advancement of regenerative medicine, which includes research and product development in the fields of biomaterials, tissue engineering and cell therapy. With more than 70 patents acquired over the last decade, my BMT lab combines knowledges and methods of biochemistry, cell biology, molecular biology, chemistry, material sciences and chemical engineering and dedicates to translate research results into industrial applications and solve the problems of clinical medicine. The core technologies in the BMT lab include production of noncontractible porous collagen matrix and high purity concentrated collagen solution, sterilization of biological materials, identification and quantification of collagen, creation of various wound dressings, novel biological adhesives, cell tissue gels, etc. to facilitate tissue regeneration. We have established a platform technology of porous collagen-based matrices to serve as tissue scaffolds which are biocompatible as well as biodegradable and can promote the process of tissue regeneration. We have found neovascularization in our invented matrices both in vitro and in vivo. With full-thickness wounds filled with matrices on the dorsal skin of guinea pigs, results demonstrated that our porous collagen-based matrices are more effective to facilitate wound healing with better quality than current best-sale clinical products. We now aim at the development of series of medical devices, such as artificial skin, novel wound dressing, biomimetic bone, etc. For the application of artificial tissue scaffolds in regenerative medicine, we also work on the plasticity of stem cells in extracellular microenvironment. Adipose tissue derived stem cells and placenta derived mesenchymal stem cells are studied on their proliferation, differentiation and morphogenesis in our matrices. Microarray and proteomics technologies are applied to investigate the signal transduction and interaction mechanism of the scaffold matrix with cells. We first published that hyaluronan kept human placenta derived-mesenchymal stem cells in a slow cell cycling mode similar to quiescence, the pristine state of stem cells in vivo, and prevented stem cells from senescence. By differential proteomic analysis, we found that associated with prominent biochemical metabolism and mitochondria functions. We also reviewed and proposed molecular mechanism of extrinsic factors affecting anti-aging of stem cells. Since human is the application target of various biomaterials and medical devices, a suitable animal model is crucial for the pre-clinical study and key to a successful translational research. Lanyu mini-pig is an endemic species in Taiwan and is promoted to be an international research animal due to its similarity to human being in body size, body weight, growth curve, physiological status, organs, tissues, etc. Besides Lanyu pigs can be handled and managed easily, our study demonstrated them to be a better model system with similar human wound healing profiles. In addition, the healing process as well as remodeling phase were similar between Lanyu and Landrace pigs, a well-known skin wound healing model. Our study also provides a systematic information on the biological changes of skin during the maturation process of Lanyu pig, which resemble significant changes of human skin properties from infants to elders. We have also successfully established a diabetic Lanyu pig model, skin wound healing model, bone fracture model, ischemic heart model and hind limb severe ischemia model for translational research.
|當選Fellow, Biomaterials Science and Engineering (FBSE)||2008.05|